α ugcg  (Bioss)

Journal: The Journal of Biological Chemistry

Article Title: Glycolipid transfer protein modulates vesicular trafficking from the endoplasmic reticulum in HeLa cells

doi: 10.1016/j.jbc.2026.111378

Figure Lengend Snippet: Cells do not compensate for the lack of GLTP by increasing levels of UGCG or LTPs, although protein localizations are altered in the absence of GLTP . A , Western blot of analysis of the levels of UGCG, FAPP2 and CERT in WT HeLa, GLTP KO, GLTP rescue and ΔFFAT-GLTP HeLa cells. Antibodies used are described in the Materials section. Beta-actin was used as a loading control. The protein band in the upper blot that runs between FAPP2, and beta-actin is unidentified. B , representative fluorescence images of coimmunostaining of glucosylceramide synthase UGCG ( green ) and Golgi marker golgin-97 ( red ) in HeLa WT cells and, ( C ) GLTP KO HeLa cells. D , immunostaining of UGCG ( green ) and golgin-97 ( red ) in GLTP rescue HeLa cells and ( E ) ΔFFAT-GLTP expressing HeLa cells. The scale bar represents 200 μm. The subpanels are higher magnifications (2x) of representative cells. CERT, ceramide transport protein; FAPP2, four-phosphate adaptor protein 2; UGCG, UDP-glucose ceramide glucosyltransferase; GLTP, glycolipid transfer protein; GLTP KO, GLTP knockout.

Article Snippet: The primary commercial antibodies used were α-Sec12 (rabbit, AB181212 ; Abcam), α-Sec16 (rabbit, HPA005684; Sigma-Aldrich), α-Sec23 A (goat, PA5-19011; Invitrogen), α-Sec23 B (mouse, MA5-27262; Invitrogen), α-Sec31 A (rabbit, PA5-52147; Invitrogen), α-UGCG (rabbit, bs-21562R; Bioss Antibodies), α-Sar1A (rabbit, PA5-103872; Invitrogen), α-CERT (rabbit, AB151285 ; Abcam), α-FAPP2 (goat, AB38748; Abcam), α-Golgin97 (mouse, A21270; Invitrogen) α-glucosylceramide (rabbit, RAS_0010; Glycobiotech GmbH), and α-galactosylceramide (rabbit, RAS_0030; Glycobiotech GmbH).

Techniques: Western Blot, Control, Fluorescence, Marker, Immunostaining, Expressing, Knock-Out

Journal: International journal of molecular sciences

Article Title: Integrating Machine Learning and Bulk and Single-Cell RNA Sequencing to Decipher Diverse Cell Death Patterns for Predicting the Prognosis of Neoadjuvant Chemotherapy in Breast Cancer.

doi: 10.3390/ijms26083682

Figure Lengend Snippet: Figure 12. The prognostic model is associated with the response to NAC in BRCA. (A,B) Pharma- cological analysis. Bubble plot of the relationship between drugs and model genes in the GDSC database (A) and CTRP database (B). (C) IHC staining of UGCG, TNFRSF21, MYB, and BTG2 in biopsy tissues of patients with different RCB grades before NAC. Scale bar = 100 µm. Abbrevia- tions: NAC, neoadjuvant chemotherapy; BRCA: breast cancer; GDSC, genomics of drug sensitivity in cancer; CTRP, cancer therapeutics response portal; IHC, immunohistochemistry; RCB, residual cancer burden.

Article Snippet: Following this, the sections were incubated overnight at 4 ◦C with primary antibodies targeting BTG (rabbit, 1:100, Proteintech (Wuhan, China), 22339-1-AP), DR6 (E-4) (also named TNFRSF21, mouse, 1:100, Santa Cruz (CA, USA), sc-376873), UGCG (rabbit, 1:100, Proteintech (Wuhan, China), 12869-1-AP), and MYB (rabbit, ready-to-use, Tongling Biomedical (Xiamen, China), AR0841).

Techniques: Immunohistochemistry

Journal: Molecular Medicine

Article Title: Miglustat ameliorates isoproterenol-induced cardiac fibrosis via targeting UGCG

doi: 10.1186/s10020-025-01093-w

Figure Lengend Snippet: The antibodies for Western Blot

Article Snippet: UGCG , Santa Cruz Biotechnology , sc-293235.

Techniques: Western Blot